Title :
Assayed Sperm Damnification by SCGE and Its Correlation with Sperm Motility and Sperm Malformation Rate
Author :
Xue Baigong ; Yao zhanfei
Author_Institution :
Norman Bethune Coll. of Med., Jilin Univ., Changchun, China
Abstract :
Objectives: To investigate the role played by sperm damnification in reproduction and correlation with sperm motility and sperm malformation rate. Method: We chosed 186 clinical samples for assaying their semen parameters by Computer Assisted Semen Analysis (CASA), meanwhile, we investigated sperm DNA damnification by Single cell gel electrophoresis(SCGE). Our work highlight that the correlation analysis of DNA damnification with the sperm deformity and motility. Then, we randomly selected some datas made statistics analysis by SPSS13.0. Result: The results showed that the SCGE method is very accurate for detected sperm DNA damnification. Statistics analysis showed that compared with the control group (male fertility), sperm viability, DNA damnification and morphology are significant difference of the group low sperm motility (P <;0.05).
Keywords :
DNA; bioelectric phenomena; bioinformatics; cell motility; correlation methods; electrophoresis; statistical analysis; Computer Assisted Semen Analysis; DNA morphology; SCGE; assayed sperm damnification; correlation analysis; single cell gel electrophoresis; sperm malformation rate; sperm motility; sperm viability; statistical analysis; Correlation; DNA; Head; Hospitals; Injuries; Morphology;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5780103
