Title :
Notice of Retraction
Optimization of cDNA-aFLP Amplification Reaction System in Strawberry Leaves
Author :
Lixiang Miao ; Yuchao Zhang ; Xiaofang Yang ; Yuejian Zhang ; Huiqin Zhang ; Guihua Jiang
Author_Institution :
Inst. of Horticulture, Zhejiang Acad. of Agric. Sci., Hangzhou, China
Abstract :
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
In this study in order to uncover the mechanism of strawberry inoculated with colletotrichum gloeosporioides and isolation the related genes, an optimized cDNA-AFLP procedure was established. The cDNA-AFLP procedures including RNA extraction, mRNA isolation reverse transcription, enzyme digestion, pre-amplification, selective amplification, silver staining. The factors affecting cDNA-AFLP analysis system with young leaves of strawberry in this study were optimized. According to the optimized reaction system, the polymorphous strips in 6% PAGE electrophoresis were clear and stable and the cDNA-AFLP reaction system developed and optimized in this experiment provided valuable information for further molecular biology and gene cloning studies on strawberry.
Keywords :
DNA; bioelectric phenomena; botany; electrophoresis; enzymes; genetics; molecular biophysics; molecular configurations; polymorphism; PAGE electrophoresis; RNA extraction; cDNA-AFLP amplification reaction system; colletotrichum gloeosporioides; complementary-DNA based amplified fragment length polymorphism; enzyme digestion; gene cloning; genes; mRNA isolation reverse transcription; molecular biology; optimization; preamplification; selective amplification; silver staining; strawberry leaves; Annealing; Gene expression; Polymers; RNA; Silver; Water;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5780128
