DocumentCode
3319457
Title
Notice of Retraction
Preliminary Purification of Salsolinol Synthase from Rat Brain
Author
Xuechai Chen ; Arshad, A. ; Xiaoyin Lin ; Yi Chen ; Jing Shen ; Yongqian Zhang ; Yulin Deng
Author_Institution
Sch. of Life Sci. & Technol., Beijing Inst. of Technol., Beijing, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Several studies suggest that 1-methyl-6, 7-dihydroxy-1, 2, 3, 4 tetrahydroisoquinoline (Salsolinol, Sal) is toxic to dopaminergic neurons, and it is one of the most discussed tetrahydroisoquinolines whose chemical structure is the same with MPTP, it may cause PD. Studies show that salsolinol is biosynthesized by a novel enzyme named salsolinol synthase in human brain. Salsolinol synthase is one kind of enzyme catalyzes the condensation of dopamine and acetaldehyde. It was preliminary purified from rat brains first time in this paper. The purification procedure involved ultracentrifugation, DEAE anion exchange chromatography and gel filtration chromatography. HPLC-ECD was used for enzyme activity detection during purification. Salsolinol synthase was purified 2753.1 fold from the crude enzyme extract.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Several studies suggest that 1-methyl-6, 7-dihydroxy-1, 2, 3, 4 tetrahydroisoquinoline (Salsolinol, Sal) is toxic to dopaminergic neurons, and it is one of the most discussed tetrahydroisoquinolines whose chemical structure is the same with MPTP, it may cause PD. Studies show that salsolinol is biosynthesized by a novel enzyme named salsolinol synthase in human brain. Salsolinol synthase is one kind of enzyme catalyzes the condensation of dopamine and acetaldehyde. It was preliminary purified from rat brains first time in this paper. The purification procedure involved ultracentrifugation, DEAE anion exchange chromatography and gel filtration chromatography. HPLC-ECD was used for enzyme activity detection during purification. Salsolinol synthase was purified 2753.1 fold from the crude enzyme extract.
Keywords
biochemistry; bioelectric phenomena; brain; catalysis; chromatography; electrochemistry; enzymes; molecular biophysics; neurophysiology; purification; 1-methyl-6, 7-dihydroxy-1, 2, 3, 4 tetrahydroisoquinoline; DEAE anion exchange chromatography; HPLC-ECD; acetaldehyde; catalysis; condensation; dopamine; dopaminergic neurons; enzyme activity detection; gel filtration chromatography; purification; rat brain; salsolinol; salsolinol synthase; tetrahydroisoquinolines; ultracentrifugation; Chemicals; Filtration; Humans; Neurons; Proteins; Purification;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5780133
Filename
5780133
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