Cloning and Analysis of LeWRKY2 Genomic DNA

Author

Yu Yongkun ; Li Na ; Sun Qingpeng

Author_Institution

Coll. of Biotechnol., Beijing Univ. of Agric., Beijing, China

fYear

2011

fDate

10-12 May 2011

Firstpage

1

Lastpage

3

Abstract

WRKY transcription factor comprised a large family of zinc-finger-type transcription factors. It involved in various biotic or abiotic stress responses and various developmental stages. Though many WRKY genes had been cloned in different kinds of species, the function and phylogenetic relationship remained unclear. In this study, a genomic DNA from tomato was cloned, bio informatics analysis revealed that it contained 3 introns and 4 extrons, and it encoded a group II WRKY transcription factor protein, named as LeWRKY2, which consisted of 156 amino acids and contained one WRKY domain, one C₂-HC type zinc finger motif. Phylogenetic analysis showed that it had the closer phylogenetic relationship with Capsicum annuum´s WRKY (Genbank no. AAW67002.1).

Keywords

DNA; biochemistry; bioinformatics; botany; genetics; genomics; molecular biophysics; LeWRKY2 genomic DNA; WRKY transcription factor protein; abiotic stress responses; bioinformatics analysis; biotic stress responses; capsicum annuum; gene cloning; genomics; phylogenetic analysis; tomato DNA; zinc-finger-type transcription factors; Amino acids; Bioinformatics; DNA; Genomics; Phylogeny; Proteins;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on

Conference_Location

Wuhan

ISSN

2151-7614

Print_ISBN

978-1-4244-5088-6

Type

conf

DOI

10.1109/icbbe.2011.5780144

Filename

5780144