DocumentCode
3321157
Title
Notice of Retraction
The Role of Listeria monocytogenes sigmaB and PrfA in the Expression of Bile Salt Hydrolase Gene bsh
Author
Qiang Zhang ; Xiaoqin Feng ; Yingying Feng ; Li Wang ; Feifei Feng ; Xiaojiao Yin ; Qingchun Zhou ; Qin Luo
Author_Institution
Hubei Key Lab. of Genetic Regul. & Integrative Biol., Central China Normal Univ., Wuhan, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Bile salt hydrolase in Listeria monocytogenes is only encoded by bsh gene. It plays an important role in hydrolyzing heavy concentration of bile salt when L. monocytogenes in the hypoxemic condition and is propitious to L. monocytogenes surviving in the host cell. There are complete SigB and PrfA binding sites in bsh promoter: PrfA-box(TTAAAAATTTTT AA) , SigB-box (GTTT-N15-GGGTAC). In order to investigate the role of SigB (σB) and PrfA in regulating bsh expression, we used RT-PCR method to analyze the bsh expression level in the four strains: L. monocytogenes EGD, EGDΔprfA, EGDΔsiB and EGDΔprfAΔsigB. The results were shown that σB and PrfA were both contribute to bsh expression, but the deletion of sigB would remarkably decrease the bsh expression, indicating σB was more important than PrfA in resistance of bile salt environment in gastrointestinal tract. This work will provide the theory evidence on the regulation mechanisms of virulence gene in gastrointestinal tract.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Bile salt hydrolase in Listeria monocytogenes is only encoded by bsh gene. It plays an important role in hydrolyzing heavy concentration of bile salt when L. monocytogenes in the hypoxemic condition and is propitious to L. monocytogenes surviving in the host cell. There are complete SigB and PrfA binding sites in bsh promoter: PrfA-box(TTAAAAATTTTT AA) , SigB-box (GTTT-N15-GGGTAC). In order to investigate the role of SigB (σB) and PrfA in regulating bsh expression, we used RT-PCR method to analyze the bsh expression level in the four strains: L. monocytogenes EGD, EGDΔprfA, EGDΔsiB and EGDΔprfAΔsigB. The results were shown that σB and PrfA were both contribute to bsh expression, but the deletion of sigB would remarkably decrease the bsh expression, indicating σB was more important than PrfA in resistance of bile salt environment in gastrointestinal tract. This work will provide the theory evidence on the regulation mechanisms of virulence gene in gastrointestinal tract.
Keywords
biochemistry; biological techniques; bonds (chemical); cellular biophysics; enzymes; genetics; microorganisms; L. monocytogenes EGD; PrfA expression; PrfA-box(TTAAAAATTTTT AA); RT-PCR method; SigB binding sites; SigB-box (GTTT-N15-GGGTAC); bile salt hydrolase gene bsh; gastrointestinal tract; hypoxemic condition; listeria monocytogenes σB expression; virulence gene; Gastrointestinal tract; Genetic expression; Immune system; Microorganisms; RNA; Strain; Stress;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5780230
Filename
5780230
Link To Document