Title :
Notice of Retraction
Osteopontin from Bovine Milk Effects on Osteoblasts of Rats In Vitro
Author :
Jie Sun ; Guoyou Yin
Author_Institution :
Biol. Eng. Dept., Henan Univ. of Urban Constr., Pingdingshan, China
Abstract :
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
To assess the effects of osteopontin on osteoblast, Large quantity of high-purity osteoblasts had been cultivated successfully by adopting sequential enzyme digestion. The growth curve of osteoblasts was protracted by cell counting. Proliferation of osteoblasts was assessed by MTT colorimetry. Alkaline phosphatase (ALP) activity test kits was carried out to evaluate the effect of osteopontin on rat osteoblasts. The experimental results show that the osteopontin can enhance proliferation, the ALP activity of osteoblast, the properties of adhesion and discuss the effect of osteopontin on osteoblast. Keywords-osteopontin; osteoblast; MTT; alkaline phosphatase.
Keywords :
biological techniques; cellular biophysics; molecular biophysics; proteins; ALP activity; MTT colorimetry; alkaline phosphatase activity; bovine milk; cell counting; high purity osteoblasts; in vitro rat osteoblasts; osteoblast growth curve; osteoblast proliferation; osteopontin effects; sequential enzyme digestion; Adhesives; Bones; Bovine; Dairy products; In vitro; Proteins; Sun;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5780262
