DocumentCode
3321923
Title
Notice of Retraction
Optimization the Protoplast Formation and Regeneration Conditions of Kluyveromyces marxianus and Saccharomyces cerevisiae
Author
Xuewu Guo ; Ruisheng Wang ; Yin Wang ; Yefu Chen ; Liping Du ; Cuiying Zhang ; Dongguang Xiao
Author_Institution
Key Lab. of Ind. Microbiol., Tianjin Univ. of Sci. & Technol., Tianjin, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
In this paper, S. cerevisiae AY-5 and K. marxianus TY-3 were used as starting strains and conditions for protoplast formation and regeneration were investigated, including snailase concentration, enzymatic temperature and treatment time. To S. cerevisiae AY-5 and K. marxianus TY-3, the most suitable protoplast formation and regeneration conditions were gained by single factors tests, snailase concentration, enzymatic temperature and treatment time were 0.5%, 24°C, 60min and 0.5%, 26°C, 60min, respectively. Subsequently, the optimum protoplast formation and regeneration conditions of S. cerevisiae AY-5 were snailase concentration 0.5%, enzymatic temperature 22°C and treatment time 90min, of K. marxianus TY-3 were snailase concentration 0.5%, enzymatic temperature 26 °C and treatment time 30 min, which were determined with the orthogonal matrix method. Under above conditions, protoplast formation and regeneration rate of S. cerevisiae AY-5 and K. marxianus TY-3 were 97.5%, 17.4% and 99.1%, 51.5%, respectively.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
In this paper, S. cerevisiae AY-5 and K. marxianus TY-3 were used as starting strains and conditions for protoplast formation and regeneration were investigated, including snailase concentration, enzymatic temperature and treatment time. To S. cerevisiae AY-5 and K. marxianus TY-3, the most suitable protoplast formation and regeneration conditions were gained by single factors tests, snailase concentration, enzymatic temperature and treatment time were 0.5%, 24°C, 60min and 0.5%, 26°C, 60min, respectively. Subsequently, the optimum protoplast formation and regeneration conditions of S. cerevisiae AY-5 were snailase concentration 0.5%, enzymatic temperature 22°C and treatment time 90min, of K. marxianus TY-3 were snailase concentration 0.5%, enzymatic temperature 26 °C and treatment time 30 min, which were determined with the orthogonal matrix method. Under above conditions, protoplast formation and regeneration rate of S. cerevisiae AY-5 and K. marxianus TY-3 were 97.5%, 17.4% and 99.1%, 51.5%, respectively.
Keywords
biological techniques; biotechnology; cellular biophysics; enzymes; microorganisms; K. marxianus TY-3; Kluyveromyces marxianus; S. cerevisiae AY-5; Saccharomyces cerevisiae; enzymatic temperature; protoplast formation; protoplast regeneration conditions; single factors tests; snailase concentration; temperature 22 degC; temperature 24 degC; temperature 26 degC; time 30 min; time 60 min; time 90 min; treatment time; Arrays; Dairy products; Ethanol; Optimization; Strain; Temperature;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5780267
Filename
5780267
Link To Document