Author_Institution :
Key Lab. of Ind. Microbiol., Tianjin Univ. of Sci. & Technol., Tianjin, China
Abstract :
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
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In this paper, S. cerevisiae AY-5 and K. marxianus TY-3 were used as starting strains and conditions for protoplast formation and regeneration were investigated, including snailase concentration, enzymatic temperature and treatment time. To S. cerevisiae AY-5 and K. marxianus TY-3, the most suitable protoplast formation and regeneration conditions were gained by single factors tests, snailase concentration, enzymatic temperature and treatment time were 0.5%, 24°C, 60min and 0.5%, 26°C, 60min, respectively. Subsequently, the optimum protoplast formation and regeneration conditions of S. cerevisiae AY-5 were snailase concentration 0.5%, enzymatic temperature 22°C and treatment time 90min, of K. marxianus TY-3 were snailase concentration 0.5%, enzymatic temperature 26 °C and treatment time 30 min, which were determined with the orthogonal matrix method. Under above conditions, protoplast formation and regeneration rate of S. cerevisiae AY-5 and K. marxianus TY-3 were 97.5%, 17.4% and 99.1%, 51.5%, respectively.
Keywords :
biological techniques; biotechnology; cellular biophysics; enzymes; microorganisms; K. marxianus TY-3; Kluyveromyces marxianus; S. cerevisiae AY-5; Saccharomyces cerevisiae; enzymatic temperature; protoplast formation; protoplast regeneration conditions; single factors tests; snailase concentration; temperature 22 degC; temperature 24 degC; temperature 26 degC; time 30 min; time 60 min; time 90 min; treatment time; Arrays; Dairy products; Ethanol; Optimization; Strain; Temperature;
