Real-Time Detection of Loop-Mediated Isothermal Amplification Reaction on Microfluidic Chip

Author

Xu, Ling-Yan ; Jin, Wei ; Zhu, Qiang-Yuan ; Yang, Wen-Xiu ; Wu, Qing-Qing ; Jin, Qin-Han ; Mu, Ying

Author_Institution

State Key Lab. of Ind. Control Technol., Zhejiang Univ., Hangzhou, China

fYear

2011

fDate

10-12 May 2011

Firstpage

1

Lastpage

3

Abstract

A device have been developed for monitoring the process of DNA loop-mediated isothermal amplification (LAMP) reaction through absorbance measurement. The LAMP reaction has a high efficiency and can synthesize large amounts of DNA in a short period of time. As its by-product pyrophosphate ions can be combined with magnesium ions, forming a white precipitate of magnesium pyrophosphate. The change of sample absorbance can be detected on line by an optic sensor. We can not only determine whether the LAMP reaction happened or not by the difference of absorbance between the start point and the end point but also monitor the dynamics process of LAMP reaction.

Keywords

DNA; biochemistry; microfluidics; molecular biophysics; DNA loop-mediated isothermal amplification reaction; LAMP reaction; absorbance measurement; microfluidic chip; optic sensor; pyrophosphate ion; real-time detection; DNA; Isothermal processes; Microfluidics; Monitoring; Optical fiber sensors; Optical fibers; Real time systems;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on

Conference_Location

Wuhan

ISSN

2151-7614

Print_ISBN

978-1-4244-5088-6

Type

conf

DOI

10.1109/icbbe.2011.5780331

Filename

5780331