Application of a flow type quartz crystal microbalance immunosensor for detection of cartilage oligomeric matrix protein and α1-antitrypsin

Author

Chi-Yen Shen ; Yu-Chen Chiu ; Ke-Nung Huang ; Shen-Li Fu ; Rey-Chue Hwang

Author_Institution

Dept. of Electr. Eng., I-Shou Univ. Kaohsiung, Kaohsiung, Taiwan

fYear

2013

fDate

23-24 Dec. 2013

Firstpage

712

Lastpage

715

Abstract

Osteoarthritis (OA) is a chronic disease that often occurs in the elderly population. Currently, the most common methods for diagnosing OA are radiographs and enzyme-linked immunosorbent assay (ELISA) method which are specific but require specialized laboratory facilities and highly trained personal. In this work, it developed a user friendly and non-invasive method that is quartz crystal microbalance (QCM) immunosensor, which the biointerface was designed to immobilize antibody by self-assembled monolayer (SAM) technique on the QCM surface. Cartilage oligomeric matrix protein (COMP) and α1-antitrypsin (AAT) were used as antibodies. The developed QCM-based immunosensor for COMP and AAT detection showed good sensitivity, linearity (99.9%), specificity and reliability.

Keywords

biochemistry; biosensors; bone; chemical sensors; diseases; molecular biophysics; monolayers; obstetrics; patient diagnosis; proteins; quartz crystal microbalances; self-assembly; α1-antitrypsin detection; ELISA method; QCM immunosensor; QCM surface; QCM-based immunosensor; SAM technique; antibody immobilization; biointerface design; cartilage oligomeric matrix protein detection; chronic disease; elderly population; enzyme-linked immunosorbent assay; flow type quartz crystal microbalance immunosensor application; noninvasive method; osteoarthritis diagnosis; radiographs; self-assembled monolayer technique; user friendly method; Biomarkers; Crystals; Degradation; Electrodes; Frequency measurement; Immune system; Proteins; α1-antitrypsin; cartilage oligomeric matrix protein; immunosensor; quartz crystal microbalance;

fLanguage

English

Publisher

ieee

Conference_Titel

Instrumentation and Measurement, Sensor Network and Automation (IMSNA), 2013 2nd International Symposium on

Conference_Location

Toronto, ON

Type

conf

DOI

10.1109/IMSNA.2013.6743375

Filename

6743375