Title :
Enhancement of Photodynamic Therapy Induced Cytotoxicity in K562 Cells by Genistein
Author :
Sun, Dan ; Zhang, Su-juan ; Wei, Yong-Feng ; Yin, Ling-fang
Author_Institution :
Nat. Key Lab. of Photoelectric Technol. & Functional Mater., Northwest Univ., Xi´´an, China
Abstract :
Genistein (GEN) is one of the major soy isoflavones. It is particularly effective in quenching free radicals generated by toxic agents. On account of its anti-oxidant property, it may protect cancer cells from free radical damage in photodynamic (PDT) during which reactive oxygen species (ROS) production was stimulated leading to irreversible tumor cell injury. In this study, the influence of genistein on K562 cells in 5-aminolevulinic acid (ALA)-based PDT is demonstrated The results showed that genistein inhibits cell proliferation and enhances cell apoptosis in ALA-PDT on K562 cells. NaN3, a singlet oxygen quencher, suppresses the genistein-induced cell apoptosis. In conclusion, genistein enhances the PDT-induced cytotoxicity in K562 cells and singlet oxygen was involved in this process.
Keywords :
cellular biophysics; free radicals; photodynamic therapy; quenching (thermal); toxicology; tumours; 5-aminolevulinic acid; K562 Cells; antioxidant property; cancer cells; cell apoptosis; cell proliferation; cytotoxicity; free radical damage; genistein; oxygen quencher; photodynamic therapy; quenching free radicals; reactive oxygen species production; soy isoflavones; toxic agents; Cancer; Fluorescence; Humans; In vitro; Medical treatment; Radiation effects; Tumors;
Conference_Titel :
Photonics and Optoelectronics (SOPO), 2011 Symposium on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-6555-2
DOI :
10.1109/SOPO.2011.5780608
