DocumentCode :
3329841
Title :
Elastic atlas registration of β- autoradiograms using scattered data interpolators
Author :
Cabello, J. ; Metaxas, A. ; Bailey, A. ; Kitchen, I. ; Wells, K.
fYear :
2009
fDate :
Oct. 24 2009-Nov. 1 2009
Firstpage :
3700
Lastpage :
3704
Abstract :
Autoradiography is a widely extended pre-clinical nuclear imaging modality used in life sciences to investigate and localise radiolabelled biological pathways in thin ex-vivo tissue sections. After the tissue section has been exposed to an ionising radiation detector the resulting labelled regions are subsequently analysed. Typically, the resulting autoradiograms are analysed manually by an expert life scientists using a visual template as reference to measure the different radioligand uptake levels in the different areas of, in our case, mouse brain. This process is extremely time consuming and error prone, with the expertise of the life scientist playing a significant role. In this paper we describe a semi-automatic method to register a template brain atlas on to the brain autoradiogram making the analysis process more efficient, repeatable and independent of the expertise of the life scientist. The method first identifies those regions with high and low level of radioligand uptake by region growing segmentation. Subsequently, the counterpart regions in the corresponding atlas image are manually identified. Finally a set of control points is extracted from each region contour in the autoradiogram and the atlas image to apply a scattered data interpolator.
Keywords :
biological tissues; brain; image registration; image segmentation; interpolation; medical image processing; radioisotope imaging; autoradiography; brain autoradiogram; elastic atlas registration; ionising radiation detector; localise radiolabelled biological pathways; preclinical nuclear imaging; radioligand uptake levels; region growing segmentation; scattered data interpolators; template brain atlas; thin ex-vivo tissue section; Ã\x9f-autoradiograms; Area measurement; Biological tissues; Biology; Brain; Image segmentation; Ionizing radiation; Mice; Nuclear imaging; Radiation detectors; Scattering; Atlas-based registration; Autoradiography; Elastic registration; Scattered data interpolators;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nuclear Science Symposium Conference Record (NSS/MIC), 2009 IEEE
Conference_Location :
Orlando, FL
ISSN :
1095-7863
Print_ISBN :
978-1-4244-3961-4
Electronic_ISBN :
1095-7863
Type :
conf
DOI :
10.1109/NSSMIC.2009.5401863
Filename :
5401863
Link To Document :
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