Title :
Comb interdigitated arrays (IDA) electrodes for more rapid and sensitive bead-based immunoassay
Author :
Kim, Sane Kwng ; Thomas, Jennifer H. ; Hesketh, Peter I. ; Li, Changming ; Halsall, Brian H. ; Heineman, William R.
Author_Institution :
Sch. of Mech. Eng., Georgia Inst. of Technol., Atlanta, GA, USA
Abstract :
This research is dedicated to the development of electrode arrays suitable for a more sensitive and faster electrochemical immunoassay system Signal amplification is achieved through coupling the redox cycling of interdigitated array (IDA) electrodes with an enzyme label, β-galactosidase. Comb IDAs enhanced the signal 3 times higher than coplanar IDAs. A magnetic bead-based enzyme assay, as a simulation of an immunoassay, has been performed using the comb and coplanar IDAs for detection. Comb IDAs enabled positioning the beads and attached enzyme closer to the sensing electrodes (∼10 gm). Detection time was less than 1 min. and the limit of detection was 70 amole of β-galactosidase.
Keywords :
biosensors; chemical analysis; immunity testing; interdigital transducers; microsensors; proteins; β-galactosidase; 1 min; comb interdigitated arrays electrodes; electrochemical immunoassay system; enzyme label; magnetic bead-based enzyme assay; rapid sensitive bead-based immunoassay; redox cycling; signal amplification; Biochemistry; Biomedical engineering; Chemical technology; Couplings; Electrodes; Electronic mail; Fabrication; Immune system; Mechanical engineering; Telephony;
Conference_Titel :
Micro Electro Mechanical Systems, 2003. MEMS-03 Kyoto. IEEE The Sixteenth Annual International Conference on
Print_ISBN :
0-7803-7744-3
DOI :
10.1109/MEMSYS.2003.1189779
