Dielectrophoretic manipulation of avidin and DNA

Author

Bakewell, David J G ; Hughes, Michael P. ; Milner, Joel J. ; Morgan, Hywel

Author_Institution

Bioelectron. Res. Centre, Glasgow Univ., UK

Volume

2

fYear

1998

fDate

29 Oct-1 Nov 1998

Firstpage

1079

Abstract

This paper demonstrates the application of dielectrophoresis (DEP) to the manipulation of biological macromolecules such as avidin and plasmid DNA. We report, for the first time, observations of negative dielectrophoresis of these macromolecules which can be used to perform stable trapping. The basic electrokinetic theory and experimental arrangements for realising DEP in microelectrode structures are described. The paper discusses our observations on the DEP manipulation of these macromolecules

Keywords

Brownian motion; DNA; biological techniques; biotechnology; electrokinetic effects; electrophoresis; molecular biophysics; proteins; Brownian motion; avidin; biological macromolecules; dielectrophoretic manipulation; electrokinetic theory; microelectrode structures; negative dielectrophoresis; plasmid DNA; polyelectrolytes; stable trapping; Biology; DNA; Dielectrophoresis; Electrodes; Electrokinetics; Microelectrodes; Molecular biophysics; Nonuniform electric fields; Polarization; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 1998. Proceedings of the 20th Annual International Conference of the IEEE

Conference_Location

Hong Kong

ISSN

1094-687X

Print_ISBN

0-7803-5164-9

Type

conf

DOI

10.1109/IEMBS.1998.745640

Filename

745640