Title :
Induction of apoptosis and G2/M cell cycle arrest by myricetin in human hepatoma HepG2 cells
Author :
Xiu-Juan, Zhang ; Qing-Ling, Huang ; Yu-Bin, Ji
Author_Institution :
Center of R&D on Life Sci. & Environ. Sci., Harbin Univ. of Commerce, Harbin, China
Abstract :
The article is about induction of apoptosis in human hepatoma HepG2 cells by myricetin (MYR). The human hepatoma HepG2 cells was treated with different doses myricetin for 72 h. Cytotoxicity was evaluated by SRB assay, apoptosis-related alterations in morphology ascertained by cytochemical staining(Hoechst 33258) and transmission electron microscopy (TEM). The cell cycle and apoptotic rate were quantified by flow cytometry (FCM).
Keywords :
biochemistry; cancer; cellular biophysics; drugs; liver; transmission electron microscopy; tumours; G2/M cell cycle arrest; SRB assay; cell apoptosis; cytochemical staining; cytotoxicity; flow cytometry; human hepatoma HepG2 cell; myricetin drug; time 72 h; transmission electron microscopy; Atmosphere; Bovine; Business; Drugs; Educational programs; Humans; Morphology; Research and development; Transmission electron microscopy; Tumors;
Conference_Titel :
IT in Medicine & Education, 2009. ITIME '09. IEEE International Symposium on
Conference_Location :
Jinan
Print_ISBN :
978-1-4244-3928-7
Electronic_ISBN :
978-1-4244-3930-0
DOI :
10.1109/ITIME.2009.5236242
