Preliminary study of salivary proteome on the diagnosis for discrimination of gastric cancer and chronic gastritis

Author

Wu, Zheng-Zhi ; Wang, Ji-Guo ; Zhang, Xiao-Li ; Cao, Mei-Qun

Author_Institution

First Affiliated Hosp. of Shenzhen Univ., Shenzhen, China

Volume

1

fYear

2009

fDate

14-16 Aug. 2009

Firstpage

70

Lastpage

75

Abstract

Objective: To investigate and seek the special biomarkers of the salivary protein expression profile for the discrimination diagnosis between gastric cancer and chronic gastritis. Method: The saliva collected were detected by applying WCX magnetic beads and using MALDI-TOF-MS technique. Corresponding peptide mass fingerprint was obtained and differential diagnostic cast was developed. Result: Differences in 77 protein peaks were observed from chronic gastritis and gastric cancer patients. One protein peak among them presented statistical significant difference (p < 0.05). Predictive model for discrimination of gastric cancer and chronic gastritis was developed. Its identification rate was 83.54%, and its capability of predication was 60.23%. Conclusion: The protein expression profile of saliva collected from gastric cancer and chronic gastritis patients was preliminarily obtained. The diagnostic cast for discrimination of the two diseases was classified by the expression of molecular weight 6021.72 Da protein.

Keywords

biochemistry; bioinformatics; biological organs; biomagnetism; cancer; medical diagnostic computing; molecular weight; proteins; proteomics; statistical analysis; time of flight mass spectra; tumours; MALDI-TOF-MS technique; WCX magnetic beads; bioinformatics; biomarkers; chronic gastritis diagnosis; discrimination diagnosis; gastric cancer diagnosis; matrix assisted laser desorption ionization time of flight mass spectrometry technique; peptide mass fingerprint; protein molecular weight; salivary protein expression profile; salivary proteome; statistical analysis; stomach; Atrophy; Biomarkers; Cancer; Diseases; Hospitals; Mass spectroscopy; Medical diagnostic imaging; Peptides; Proteins; Temperature; MALDI-TOF-MS; bioinformatics; chronic gastritis; diagnostic cast; gastric cancer; proteomics; saliva;

fLanguage

English

Publisher

ieee

Conference_Titel

IT in Medicine & Education, 2009. ITIME '09. IEEE International Symposium on

Conference_Location

Jinan

Print_ISBN

978-1-4244-3928-7

Electronic_ISBN

978-1-4244-3930-0

Type

conf

DOI

10.1109/ITIME.2009.5236460

Filename

5236460