Title :
Notice of Retraction
Expression and Antibody Production of the NP1 and VP1u Gene from Human Bocavirus
Author :
Jinfeng Ouyang ; Jie Xiao ; Hu Han ; Jingjing Li ; Bin Sun ; Sheng Ding ; Yongbo Yang ; Yi Li
Author_Institution :
Inst. of Entomology, Hua Zhong Normal Univ., Wuhan, China
Abstract :
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
The nonstructural gene NP1 and structural gene VP1u of human Bocavirus, a newly found etiological parvovirus of lower respiratory tract infection in children, were cloned and expressed as MBP-NP1 and MBP-VP1u fusion proteins in Escherichia coli. The recombinant proteins were purified by Amylose affinity chromatography and then cleaved by the Factor Xa to remove the tag. MBP Tag-free proteins were used to immunize the New Zealand white rabbit or white mouse respectively to make antibodies, which along with the purified proteins provide reliable tools for diagnosis and the study of the virus.
Keywords :
biochemistry; cellular biophysics; chromatography; diseases; enzymes; genetics; genomics; microorganisms; molecular biophysics; molecular configurations; patient diagnosis; physiological models; MBP tag-free proteins; amylose affinity chromatography; antibodies; antibody production; escherichia coli; etiological parvovirus; fusion proteins; gene cloning; gene expression; human bocavirus; immunization; lower respiratory tract infection; nonstructural gene NP1; patient diagnosis; recombinant proteins; structural gene VP1u; white mouse model; white rabbit model; DNA; Humans; Mice; Production; Proteins; Purification; Rabbits;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5781266
