DocumentCode
3342982
Title
Notice of Retraction
Enzymatic Conversion of 2,4-Dichlophenol by Laccase
Author
Huifang Xie ; Linguo Zhao ; Weibin Chen
Author_Institution
Coll. of Chem. Eng., Nanjing Forestry Univ., Nanjing, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Laccase from Coriolus versicolor was used to study the conversion of 2,4-Dichlorophenol (DCP). DCP can be transformed nearly half after treating by 0.1ΔA/mL for 2h, and the conversion ratio DCP reached above 80% after treating for 5h when the initial DCP was 80mg/L, pH 6.5, temperature 35°C High DCP concentration will inhibit the conversion. The existents of ethanol, dioxane and acetone decreased the DCP conversion. But conversion ratio was still above 50% after 4h and above 65% after 7h, which implied that when the substrates were poorly soluble in water, the organic solvents can be chosen to increase their solubility and make their enzymatic conversion possible. UV, FTIR and HPLC spectra were used to analyze the conversion production, and results showed that DCP was degraded by multistep pathway involving the formation of quinonic structure and/or tetrahydroxybenzene, catechol and/or chlorocatechol, the cleavage of aromatic ring and so on.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
Laccase from Coriolus versicolor was used to study the conversion of 2,4-Dichlorophenol (DCP). DCP can be transformed nearly half after treating by 0.1ΔA/mL for 2h, and the conversion ratio DCP reached above 80% after treating for 5h when the initial DCP was 80mg/L, pH 6.5, temperature 35°C High DCP concentration will inhibit the conversion. The existents of ethanol, dioxane and acetone decreased the DCP conversion. But conversion ratio was still above 50% after 4h and above 65% after 7h, which implied that when the substrates were poorly soluble in water, the organic solvents can be chosen to increase their solubility and make their enzymatic conversion possible. UV, FTIR and HPLC spectra were used to analyze the conversion production, and results showed that DCP was degraded by multistep pathway involving the formation of quinonic structure and/or tetrahydroxybenzene, catechol and/or chlorocatechol, the cleavage of aromatic ring and so on.
Keywords
Fourier transform spectra; biotechnology; chemical engineering; enzymes; infrared spectra; organic compounds; solubility; solvents (industrial); ultraviolet spectra; wastewater treatment; 2,4-dichlophenol; Coriolus versicolor; FTIR spectra; HPLC spectra; Laccase; UV spectra; acetone; chlorocatechol; dioxane; enzymatic conversion; ethanol; organic solvents; quinonic structure; solubility; temperature 35 degC; tetrahydroxybenzene; time 5 h; Biochemistry; Compounds; Degradation; Ethanol; Infrared spectra; Solvents; Substrates;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5781380
Filename
5781380
Link To Document