Title :
Notice of Retraction
Kinetic Studies for the Inhibition Effect of 4-Chlorophenol on Acetylcholinesterase Activity
Author :
Qiong-Yu Liu ; Jun-Xia Liu
Author_Institution :
Sch. of Chem. & Environ. Eng., Jianghan Univ., Wuhan, China
Abstract :
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
The inhibition effect of 4-chlorophenol (4-CP) on acetylcholinesterase (AChE) and its kinetics were studied by spectrophotometry. The results show that the activity of AChE can be inhibited obviously by 4-CP, and the 50% inhibitory concentration (IC50) is 0.66 mmol·L-1. 4-CP acted as a mixed inhibitor of AChE, and a reversible inhibition mechanism were observed. Lineweaver-Burk plot results showed that the mixed inhibitory constants Ki and K´i were 0.55 mmol·L-1 and 1.60 mmol·L-1, respectively, which indicated that the inhibition effect of 4-CP on free enzyme was stronger than that on enzyme-substrate complex.
Keywords :
biochemistry; enzymes; molecular biophysics; spectrophotometry; 4-chlorophenol inhibition effect; Lineweaver-Burk plot; acetylcholinesterase activity; enzyme; kinetic studies; mixed inhibitory constants; reversible inhibition mechanism; spectrophotometry; Biochemistry; Humans; Inhibitors; Kinetic theory; Retina; Substrates;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5781411
