Title :
Coherent anti-Stokes Raman scattering microscopy
Author :
Holtom, G.R. ; Zumbusch, A. ; Sunney Xie, X.
Author_Institution :
William R. Wiley Environ. Molecular Sci. Lab., Pacific Northwest Lab., Richland, WA, USA
Abstract :
Summary form only given. We report a new nonlinear optical method for high resolution microscopy which is based on a well established technique, Coherent Anti-Stokes Raman Spectroscopy (CARS). The contrast mechanism for image formation depends on vibrational properties of the sample. This method is complementary to a variety of optical microscopies that have emerged recently, which use either linear or non-linear light interactions. Unlike confocal and two-photon fluorescence microscopy, native fluorescence or stain is not required, yet we share the capability of acquiring a stack of images to reconstruct a three dimensional structure.
Keywords :
coherent antiStokes Raman scattering; image reconstruction; multiwave mixing; nonlinear optical susceptibility; optical images; optical microscopy; vibrational states; CARS; coherent antiStokes Raman Spectroscopy; coherent antiStokes Raman scattering microscopy; confocal microscopy; contrast mechanism; high resolution microscopy; image formation; images; linear light interactions; native fluorescence; nonlinear light interactions; nonlinear optical method; optical microscopies; reconstruction; stain; three dimensional structure; two-photon fluorescence microscopy; vibrational properties; Frequency; Glass; Laser excitation; Laser tuning; Nonlinear optics; Optical microscopy; Optical mixing; Optical pumping; Raman scattering; Ultrafast optics;
Conference_Titel :
Quantum Electronics and Laser Science Conference, 1999. QELS '99. Technical Digest. Summaries of Papers Presented at the
Conference_Location :
Baltimore, MD, USA
Print_ISBN :
1-55752-576-X
DOI :
10.1109/QELS.1999.807132
