Computational model of DIC microscopy for reconstructing 3-D specimens: from observations to measurements

Author

Kagalwala, F. ; Kanade, T. ; Lanni, F.

Author_Institution

Robotics Inst., Carnegie Mellon Univ., Pittsburgh, PA, USA

fYear

1992

fDate

23-28 May 1992

Firstpage

35

Lastpage

36

Abstract

Summary form only given. Differential interference contrast (DIC) microscopy, a method pioneered by Georges Nomarski, is widely used to study live biological specimens. However, to date, biologists only qualitatively interpret DIC microscope images. In this work, we describe a method to extract quantitative information from optically-sectioned DIC microscope images. Specifically, given a set of images of a specimen, we attempt to reconstruct the three-dimensional structure and refractive index distribution throughout the specimen.

Keywords

biological techniques; light interference; optical microscopy; refractive index; DIC microscopy; computational model; differential interference contrast microscopy; live biological specimens; optically-sectioned DIC microscope images; reconstructing 3D specimens; refractive index distribution; three-dimensional structure; Biological system modeling; Biology computing; Biomedical optical imaging; Computational modeling; Data mining; Image reconstruction; Interference; Microscopy; Optical refraction; Optical variables control;

fLanguage

English

Publisher

ieee

Conference_Titel

Quantum Electronics and Laser Science Conference, 1999. QELS '99. Technical Digest. Summaries of Papers Presented at the

Conference_Location

Baltimore, MD, USA

Print_ISBN

1-55752-576-X

Type

conf

DOI

10.1109/QELS.1999.807134

Filename

807134