DocumentCode
3346453
Title
Notice of Retraction
Cytotoxicity of Eight Organic Solvents Towards Balb/3T3 and 293T Cells
Author
Wu Shuang ; Li Fan ; Ma Xuemei ; Wang Minglian ; Zhang Ping ; Zhong Rugang
Author_Institution
Coll. of Life Sci. & Bioeng., Beijing Univ. of Technol., Beijing, China
fYear
2011
fDate
10-12 May 2011
Firstpage
1
Lastpage
4
Abstract
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
The aim of this study is to select organic solvents that dissolve large numbers of poor water-soluble chemicals including environmental contaminants in cell transformation assay and provide reference for solvents vehicle selection. The cytotoxicity of eight commonly used organic solvents-ethanol, methanol, acetone, isopropyl alcohol, 1,2-propanediol, glycerin, acetic ether and dimethyl sulfoxide (DMSO) towards Balb/3T3 and 293T cells has been studied by CCK-8 assay. The results indicated that the cytotoxicity increased significantly with the increasing concentration of solvent compared to the control group, and it showed a dosage-response relationship. Methanol, DMSO and ethanol exhibited much less cytotoxicity, which was slightly different between the two cell lines. The concentrations with 70% of cell viability were 3.70%, 1.40% or 1.20% (v/v) in Balb/3T3 cells and 2.20%, 1.40% or 0.80% (v/v) in 293T cells, respectively. The five other solvents displayed much greater cytotoxicity, the concentrations with 70% of cell viability were much lower. The concentrations of acetic ether and acetone with 70% of cell viability were below 0.20%. While, the difference of concentrations of isopropyl alcohol, glycerin and 1,2-propanediol with 70% of cell viability was 2-4 times between the two cell lines. The cell viability percent in solvents with little cytotoxicity should be above 90%. Methanol at 0-1.0% (v/v) and DMSO at 0 0.3% (v/v) presented much less cytotoxicity towards Balb/3T3 cells, the cell viability percent was ab- ut 95%. Ethanol at 0-0.3% (v/v) showed the least cytotoxic effect towards 293T cells, the cell viability percent was approximately 95%. Therefore, methanol/DMSO and ethanol might be compatible solvents vehicle towards Balb/3T3 and 293T cells, respectively. Methanol and ethanol were much better than DMSO towards the two cell lines. In addition, the selection of solvents vehicle might be affected by cell type.
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
The aim of this study is to select organic solvents that dissolve large numbers of poor water-soluble chemicals including environmental contaminants in cell transformation assay and provide reference for solvents vehicle selection. The cytotoxicity of eight commonly used organic solvents-ethanol, methanol, acetone, isopropyl alcohol, 1,2-propanediol, glycerin, acetic ether and dimethyl sulfoxide (DMSO) towards Balb/3T3 and 293T cells has been studied by CCK-8 assay. The results indicated that the cytotoxicity increased significantly with the increasing concentration of solvent compared to the control group, and it showed a dosage-response relationship. Methanol, DMSO and ethanol exhibited much less cytotoxicity, which was slightly different between the two cell lines. The concentrations with 70% of cell viability were 3.70%, 1.40% or 1.20% (v/v) in Balb/3T3 cells and 2.20%, 1.40% or 0.80% (v/v) in 293T cells, respectively. The five other solvents displayed much greater cytotoxicity, the concentrations with 70% of cell viability were much lower. The concentrations of acetic ether and acetone with 70% of cell viability were below 0.20%. While, the difference of concentrations of isopropyl alcohol, glycerin and 1,2-propanediol with 70% of cell viability was 2-4 times between the two cell lines. The cell viability percent in solvents with little cytotoxicity should be above 90%. Methanol at 0-1.0% (v/v) and DMSO at 0 0.3% (v/v) presented much less cytotoxicity towards Balb/3T3 cells, the cell viability percent was ab- ut 95%. Ethanol at 0-0.3% (v/v) showed the least cytotoxic effect towards 293T cells, the cell viability percent was approximately 95%. Therefore, methanol/DMSO and ethanol might be compatible solvents vehicle towards Balb/3T3 and 293T cells, respectively. Methanol and ethanol were much better than DMSO towards the two cell lines. In addition, the selection of solvents vehicle might be affected by cell type.
Keywords
cellular biophysics; dissolving; organic compounds; toxicology; 1,2-propanediol; 293T cells; CCK-8 assay; acetic ether; acetone; balb-3T3; cell transformation assay; cell viability; compatible solvents; cytotoxicity; dimethyl sulfoxide; dosage-response relationship; environmental contaminants; ethanol; glycerin; isopropyl alcohol; least cytotoxic effect; methanol; organic solvents; solvent vehicle selection; water-soluble chemicals; Cancer; Chemicals; Ethanol; Methanol; Protocols; Solvents; Vehicles;
fLanguage
English
Publisher
ieee
Conference_Titel
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location
Wuhan
ISSN
2151-7614
Print_ISBN
978-1-4244-5088-6
Type
conf
DOI
10.1109/icbbe.2011.5781580
Filename
5781580
Link To Document