A microfluidic electrochemical cell based on microsystem packaging technologies applicable for biosensor development

A new miniaturized electrochemical cell has been designed and constructed by utilization of different electronics technologies. The 25-50 μl capacity of the cylindrical shaped electrochemical cell has been built up by cutting circles in 5-10 overlapping layers of 400 μm thick Teflon foil. This multilayer structure enables very flexible solutions for in- and outlet of different materials into the electrochemical cell. The number of the working electrodes of the biosensor substrates can vary between 1-80. The reference electrode has been made of a silver wire. The circular symmetry of the counter and reference electrodes and the pattern of the working electrode array allows bipotentiostatic measurements, thus, this flexible platform is suitable for wide range of experiments of both bipotentiostatic biocatalytic sensors and DNA sensors with electronically addressed immobilization.