Title :
Notice of Retraction
A Rapid, Efficient Method for the Mass Production of Viable Generative Cells from Nicotiana tabacum, Vicia faba L. and Iris tectorium Maxim
Author :
Fang Kefeng ; Yu Tongquan
Author_Institution :
Beijing Key Lab. of New Technol. in Agric. Applic., Beijing Univ. of Agric., Beijing, China
Abstract :
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
An optimized protocol was established to isolate large numbers of mature living generative cells from Nicotiana tabacum, Vicia faba L., and Iris tectorium Maxim. Fresh pollen grains on the day of anthesis were incubated in a solution containing 9% mannitol. The optimal pH and temperature for the reaction were 5.8 and 24°C, respectively. For a short time, large amount of generative cells were subsequently released into the solution and were immediately collected for preparation. The isolated pollen protoplasts were demonstrated to be living by microscopy in a fluorochromatic reaction with fluorescein diacetate (FDA). The isolation rate was above 95%.
Keywords :
biological techniques; botany; cellular biophysics; isolation technology; pH; Iris tectorium Maxim; Nicotiana tabacum; Vicia faba L; anthesis; fluorescein diacetate; fluorochromatic reaction; isolation rate; mass production; pH; pollen grains; protoplasts; viable generative cells; Agriculture; Electric shock; Fuses; Iris; Materials; Microscopy; Protocols;
Conference_Titel :
Bioinformatics and Biomedical Engineering, (iCBBE) 2011 5th International Conference on
Conference_Location :
Wuhan
Print_ISBN :
978-1-4244-5088-6
DOI :
10.1109/icbbe.2011.5781622
