Notice of RetractionGenome Cloning of Human Bocavirus (HBoV1) and Prospective Study of NP1 Gene Transcription

Notice of Retraction

After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.

We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.

The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.

Human bocavirus (HBoV) is a recently discovered parvovirus which is suspected to be an etiologic agent of respiratory disease and gastrointestinal disease in humans. In this study, We detected positive samples for HBoV1 from nasopharyngeal aspirates and constructed the nearly full-length genome clone designated as pWHL-1(GenBank Acession NO. GU139423). Furthermore, using the RT-PCR and 3´RACE method we identified different viral mRNAs of NP1 gene in 293T cells transfected by pWHL-1. The mRNAs were varied due to alternative splicing and alternative polyadenylation. Our results showed that gene transcription occurred in pWHL-1 transfected cells even without inverted terminal repeats (ITRs) structure of the viral genome.