Start-up and continuous operation of CSTR by biohybacterium R3

A continuous stirred-tank reactor (CSTR) for biohydrogen production using Biohybacterium R3 which is a new anaerobic hydrogenogens bacteria. The point was to assess the start-up and continuous operation characteristics. It was found that at pH of about 4.5, the COD concentration of 6000 mg·L^-1, and a hydraulic retention time (HRT) of 8h, a hydrogen producing microbial community could be adapt on the environment and stable operation. At the same time, the ORP of the Biohydrogen reactor system is about -400 mV, steadily; in the system, the concentration of ethanol is the highest, about 65% of the aqueous terminal production. The total hydrogen production is 399.33 L, and the highest hydrogen production is 15768.8 mL·d^-1 during the stage of the start-up and operation. The organic source of nitrogen can be used by microbial, while the inorganic nitrogen has no severely effects to hydrogen-production; when use some organic source of nitrogen and phosphorus, the accumulate gas production, the accumulation of hydrogen production and total glucose concentration on end liquid products were higher than blank.