DocumentCode :
3374463
Title :
A Microfluidic System for Automatic Cell Culture
Author :
Huang, C.W. ; Huang, S.B. ; Lee, G.-B.
Author_Institution :
Nat. Cheng Kung Univ., Tainan
fYear :
2007
fDate :
10-14 June 2007
Firstpage :
783
Lastpage :
786
Abstract :
This study presents a new chip capable of automating the cell culture process by using microfluidic technology. This microfluidic cell culture system comprising of microheaters, a micro temperature sensor, micropumps, microvalves, microchannels, a cell culture area and several reservoirs was fabricated by using micro-electro-mechanical-systems (MEMS) fabrication processes. Manual cell culture processes can be performed on this chip without using incubators. A typical cell culturing process for human lung cancer cells (A549) was successfully performed to demonstrate the capability of the developed microfluidic system. This automatic cell culturing system can be eventually integrated with subsequent microfluidic modules for cell purification, collection, counting and lysis to form a cell-based micro-total-analysis-system.
Keywords :
bioMEMS; biotechnology; cellular biophysics; microfluidics; automatic cell culture; human lung cancer cells; micro-electro-mechanical-systems; microchannels; microfluidic cell culture system; microheaters; micropumps; microtemperature sensor; microvalves; Fabrication; Humans; Lungs; Microchannel; Microfluidics; Micromechanical devices; Micropumps; Microvalves; Reservoirs; Temperature sensors; Cell culture; MEMS; Microfluidics;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location :
Lyon
Print_ISBN :
1-4244-0842-3
Electronic_ISBN :
1-4244-0842-3
Type :
conf
DOI :
10.1109/SENSOR.2007.4300247
Filename :
4300247
Link To Document :
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