Effect of He-Ne laser treatment on the level of lipid peroxidation products in experimental cataract of rabbit eyes

Summary form only given. The processes of lipid peroxidation (LPO) in cell membranes modify effectively the lipid structure of biomembranes, which in turn have an effect on the activity on the lipid-dependent membrane-bond enzyme complexes. Experimental and clinical data show that the pathogenesis of eye disease, i.e. cataract, increases the level of LPO-products. LPO is a complex process, including the generation of different products-primary products such as conjugated diens and secondary products such as malondialdehyde (MDA). The aim of this study is to evaluate the effect of low-intensity laser irradiation (He-Ne laser, Karl Zeis-Yena, 1=632 nm) on the processes of LPO in experimental diquat-induced cataract of rabbit eyes. The laser treatment was carried out for 3 minutes daily for 5 days before and 14 days after diquat administration. The irradiance on the rabbit eye was 0.1 mW/cm2. The development of cataract was followed for 9 weeks-the time for a full darkening of the lens. After enucleation the eyes were subjected to analysis of LPO-products. Our results show that the experimental model of cataract provokes an increase of the level of conjugated diens in humor aquosis by 20% and in lens homogenate by 60%, and of the content of MDA by 34% and 96%, respectively. The laser therapy did not influence the followed parameters significantly