Title :
High-Throughput Single-Cell Electroporation Microchip with Three Dimensional Si Microelectrodes for Gene Transfection
Author :
Cho, Y.H. ; Lee, S.W. ; Le Pioufle, B. ; Takama, N. ; Takeuchi, S. ; Fujii, T. ; Kim, B.J.
Author_Institution :
Univ. of Tokyo, Tokyo
Abstract :
We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.
Keywords :
electrophoresis; microelectrodes; micromechanical devices; fluorescent microscope; gene transfection; microelectrodes; single-cell electroporation microchip; Biomembranes; Cells (biology); DNA; Electrodes; Fabrication; Fluorescence; Low voltage; Microchannel; Microelectrodes; Scanning electron microscopy; Electroporation; GFP; Twin microcantilever array;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location :
Lyon
Print_ISBN :
1-4244-0842-3
Electronic_ISBN :
1-4244-0842-3
DOI :
10.1109/SENSOR.2007.4300286
