Title :
Amplification circuit and microelectrode array for HL-1 Cardiomyocyte action potential measurement
Author :
Song, Jianan ; Welch, David ; Christen, Jennifer Blain
Author_Institution :
Electr. Eng., Arizona State Univ., Tempe, AZ, USA
fDate :
May 30 2010-June 2 2010
Abstract :
We have designed and tested two amplification circuits for measuring HL-1 cardiac action potentials, single-ended and differential. We describe the improvements in performance resulting from the differential configuration. These improvements are especially important considering the cell culture´s aqueous environment that experiences DC drift. In addition we have designed and fabricated a 4×4 gold microelectrode array and cultured HL-1 cells on the electrodes. The array is connected to our circuit through a custom made Pogo pin jig to allow for easy measurements from the electrodes while cells are being cultured. This setup allowed us to obtain initial results for modeling the transfer function of the cell culture as a passive device network. Testing has confirmed the circuits are capable of filtering low frequency signals including DC drift and frequencies above 5 kHz. The amplification was achieved through two gain stages with a total gain of 60 dB for the single-ended and 46 dB for the differential circuits.
Keywords :
amplifiers; bioelectric potentials; biomedical electrodes; biomedical electronics; biomedical measurement; cardiology; cellular biophysics; differentiating circuits; microelectrodes; transfer functions; 4×4 gold microelectrode array; Au; DC drift; HL-1 cardiomyocyte; Pogo pin jig; action potential measurement; amplification circuit; differential circuits; gain 46 dB; gain 60 dB; low frequency signal filtering; transfer function; Cardiology; Cells (biology); Circuit testing; Electrodes; Filtering; Frequency; Gain; Gold; Microelectrodes; Transfer functions;
Conference_Titel :
Circuits and Systems (ISCAS), Proceedings of 2010 IEEE International Symposium on
Conference_Location :
Paris
Print_ISBN :
978-1-4244-5308-5
Electronic_ISBN :
978-1-4244-5309-2
DOI :
10.1109/ISCAS.2010.5537259