Title :
Whole-Cell Conductometric Biosensor for Determination Heavy-Metals in Water
Author :
Berezhetskyy, A.L. ; Durrieu, C. ; Chovelon, J.-M. ; Dzyadevych, S.V. ; Tran-Minh, C.
Abstract :
Whole-cell Chlorella vulgaris conductometric biosensors consisting of interdigitated gold electrodes and sol-gel algal membranes have been used for assessment of heavy-metal ions in water. These analytes act as algal alkaline phosphatase (AP) inhibitors. Enzyme residual activity has been measured in Tris-nitrate buffer in the presence of Mg2+ ions as activator. The results indicate that inhibition of AP was found for ppm concentration levels of tested metals while activation of AP activity occurs at ppb concentration levels, except for Zn2+ optimized and its characteristics are discussed.
Keywords :
biosensors; cellular biophysics; electrochemical electrodes; inhibitors; magnesium; sol-gel processing; water; Chlorella vulgaris; Mg2+; algal alkaline phosphatase inhibitors; enzyme residual activity; gold electrodes; heavy-metal ions; sol-gel algal membranes; water; whole-cell conductometric biosensor; Algae; Biochemistry; Biomembranes; Biosensors; Electrodes; Environmentally friendly manufacturing techniques; Frequency measurement; Inhibitors; Pollution measurement; Sensor phenomena and characterization; Chlorella vulgaris; conductometry; heavy-metals ion determination; inhibitor analysis; sol-gel immobilization; thin-film interdigitated electrodes;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International
Conference_Location :
Lyon
Print_ISBN :
1-4244-0842-3
Electronic_ISBN :
1-4244-0842-3
DOI :
10.1109/SENSOR.2007.4300410
