Analysis of Micrometer Sample Flows in a Non Coaxial Sheath Flow Device

Author

Hairer, G. ; Pärr, G.S. ; Svasek, P. ; Jachimowicz, A. ; Vellekoop, M.J.

Author_Institution

Vienna Univ. of Technol., Vienna

fYear

2007

fDate

10-14 June 2007

Firstpage

1845

Lastpage

1848

Abstract

In this paper we present a novel non coaxial sheath flow device with channel dimensions of 50 mum in square at the focusing region. Analyses of the adaptable sample flow are carried out where the focusing limitation and its profile variations are shown using confocal microscope measurements. A comparison of the sample flow height inside the channel and its relative fluorescence intensity is highlighted. Additionally, the influence of the hydrodynamic focusing on the sample profile and its uniformity over a certain distance is shown.

Keywords

fluidic devices; fluorescence; hydrodynamics; microchannel flow; micrometry; microscopes; confocal microscope measurements; fluorescence intensity; hydrodynamic; microfluidic; micrometer sample flows; noncoaxial sheath flow device; Actuators; Cells (biology); Coaxial components; Fluid flow measurement; Fluorescence; Focusing; Hydrodynamics; Image motion analysis; Microfluidics; Optical sensors; Microfluidics; hydrodynamic focusing; non coaxial sheath flow;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300515

Filename

4300515