Voltammetric Analysis of DNA Films Immobilized on Gold Film Electrodes Carrying Single Base Pair Mismatches

Author

Pänke, Oliver ; Lisdat, Fred

Author_Institution

Univ. of Appl. Sci. Wildau, Wildau

fYear

2007

fDate

10-14 June 2007

Firstpage

1991

Lastpage

1993

Abstract

The application of electrochemical techniques for DNA detection is motivated by their potential to detect hybridisation events in a more rapid, simplistic and cost-effective manner compared to conventional optical assays. Here, we analyzed DNA films immobilized onto gold film electrodes containing single base pair mismatches and covalently attached methylene blue (MB) by means of cyclic (CV) and differential pulse voltammetry (DPV) and determined the rate constant for the electron transfer reaction between MB moiety and electrode for different types of mismatches.

Keywords

DNA; biosensors; electrodes; voltammetry (chemical analysis); DNA films; DNA hybridisation; MB moiety; biosensor; covalently attached methylene blue; cyclic voltammetry; differential pulse voltammetry; electrochemical DNA detection; electrochemical techniques; electron transfer reaction; gold film electrodes; hybridisation events; optical assays; single base pair mismatches; voltammetric analysis; Actuators; Biomedical electrodes; Biomedical engineering; DNA; Electrons; Event detection; Gold; Optical films; Sequences; Solid state circuits; Biosensor; DNA hybridisation; differential pulse voltammetry; electrochemical DNA detection; methylene blue; single base pair mismatch;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference, 2007. TRANSDUCERS 2007. International

Conference_Location

Lyon

Print_ISBN

1-4244-0842-3

Electronic_ISBN

1-4244-0842-3

Type

conf

DOI

10.1109/SENSOR.2007.4300552

Filename

4300552