The preliminary study of lactate detection based on lactate dehydrogenase/nictotinamide adenine dinucleotide

Author

Taertulakarn, S. ; Tuantranont, Adisorn ; Tobanluepop, P. ; Pintavirooj, Chuchart

Author_Institution

Fac. of Allied Health Sci., Thammasat Univ., Pathumthani, Thailand

fYear

2012

fDate

5-7 Dec. 2012

Firstpage

1

Lastpage

3

Abstract

This study reports the preliminary development to biosensor using electrochemistry for lactate analysis. In this work, PEDOT/PSS modified screen printed carbon was developed as working electrode with lactate dehydrogenase (LDH), the oxidized form of nicotinamide adenine dinucleotide (NAD⁺) and glutaraldehyde. The result shows that the current from working electrode with PEDOT/PSS were higher compared with screen printed carbon electrode. These results were consistent with PEDOT/PSS properties that are conducting polymers and can help for more rapid electron transfer. These favorable characteristics allowed its application to detect normal blood lactate concentration in human beings.

Keywords

amperometric sensors; biochemistry; biosensors; conducting polymers; electrochemical electrodes; electrochemistry; enzymes; hydrogenation; molecular biophysics; oxidation; PEDOT-PSS modified screen printed carbon; PEDOT-PSS properties; biosensor; blood lactate concentration; conducting polymers; electrochemistry; electron transfer; glutaraldehyde; lactate analysis; lactate dehydrogenase-nictotinamide adenine dinucleotide; lactate detection; oxidation; screen printed carbon electrode; Biochemistry; Biosensors; Blood; Carbon; Electrodes; Substrates; Amperometric lactate biosensor; Lactate dehydrogenase; Lactate detection;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering International Conference (BMEiCON), 2012

Conference_Location

Ubon Ratchathani

Print_ISBN

978-1-4673-4890-4

Type

conf

DOI

10.1109/BMEiCon.2012.6465514

Filename

6465514