Microanalysis System Based on Electrochemiluminescence with Automatic Mixing and pH-Regulation Functions

In conducting a bio/chemical analysis on a chip, several different procedures are usually required. In this study, we demonstrate the possibility to conduct necessary procedures on a single chip. Our fabricated device has functions such as automatic mixing of solutions, pH-regulation, and detection based on electrochemiluminescence (ECL). A reagent solution containing Ru(bpy)₃ ²⁺ and a sample solution containing an amino acid were mixed and the solution pH was adjusted using the above- mentioned mechanisms. When a potential was applied to the electrode for ECL, red luminescence was observed. The ECL intensity increased with the increase in the concentration of amino acids.