Association analysis between erythrocyte membrane surface structure and genes in Cyprinus carpio with different vitality at lower temperature

Erythrocyte membrane surface structure in common carp (Cyprinus carpio) with different vitalities at lower temperatures was observed using atomic force microscope (AFM). The results showed that carp individuals with cold tolerance have spine heave in its erythrocyte membrane edge, whereas the counterparts haven´t this structure. Two EST markers named of CC005 and CC062 isolated from cDNA library constructed under cooling temperature was found to be connected with the presence of spine heave in erythrocyte membrane. A significance (X²=21.345, P<0.01) was detected in genotypic frequencies related to the presence of spine heave obtained simultaneously at CC005 and CC062. Thus, we inferred that polymorphisms of two ESTs have a collaborative effect on the conformation of spine heave. This study will offer supportive theoretics to conduct blood physiological research of cold adaption in fish species.