Title :
Measuring IL-6 and IL-6R Interaction on Single Living Cell Using Nano-Optical Tweezers
Author :
Cheng, Wen ; Yan, Zhang ; Anpei, Ye
Author_Institution :
Peking Univ., Beijing
fDate :
July 29 2007-Aug. 11 2007
Abstract :
The single molecular level low-affinity between IL-6 and IL-6R was obtained in vivo through the nano-optical tweezers system developed by our group. This affinity is 42.1 plusmn 11.7 pN when the loading rate is 3100 pN-nm/s and the QD accuracy reaches 1 nm.
Keywords :
biochemistry; biological techniques; cellular biophysics; molecular biophysics; nanobiotechnology; proteins; radiation pressure; interleukin; nano-optical tweezers; quadrant detector; single living cell; single molecular level low-affinity; Biomedical optical imaging; Biomembranes; Charge carrier processes; Charge coupled devices; Humans; Immune system; In vivo; Nanobioscience; Optical microscopy; Optical noise;
Conference_Titel :
Nano-Optoelectronics Workshop, 2007. i-NOW '07. International
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-1591-5
DOI :
10.1109/INOW.2007.4302936
