Title :
Notice of Retraction
Preparation of chitosan / CMC capsule and immobilized sucrase
Author :
Hou, M.W.-L. ; Yang, T. ; Zhang, Z.-W. ; Niu, S.-L. ; Liu, X.-M. ; Yang, Y.-D.
Author_Institution :
Hebei Normal Univ. of Sci. & Technol., Qinhuangdao, China
Abstract :
Notice of Retraction
After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE´s Publication Principles.
We hereby retract the content of this paper. Reasonable effort should be made to remove all past references to this paper.
The presenting author of this paper has the option to appeal this decision by contacting TPII@ieee.org.
The immobilized sucrase capsule of 3.0mm diameter was prepared by chitosan and sodium carboxymethyl cellulose (CMC) ion interaction. The immobilization conditions were optimized as following: The sucrase was dissolved in chitosan solution with the concentration of 2.0%. The solution was dropped into the CMC solution with concentration of 1.5%, and the formed capsules were kept hardening for 24h at room temperature. The immobilized enzyme more stable to pH values, the optimum pH of the immobilized sucrase for the enzyme activity was at 3.6~5.0, and the activity of free enzyme at pH = 3.6 became 63% of maximum activity. The immobilize sucrase stored at room temperature retained 80% of its initial activity even after 6d, and free enzyme retained 15% of its initial activity. The enzyme activity of the immobilized sucrase retained almost the same after repeated use, and leaching of the enzyme after 5 cycles of reuse.
Keywords :
biochemistry; biomedical materials; enzymes; leaching; materials preparation; molecular biophysics; carboxymethyl cellulose; chitosan/CMC capsule; enzyme; hardening; immobilized sucrase; leaching; size 3.0 mm; temperature 293 K to 298 K; time 24 h; Copper; chitosan; immobilization; stability; sucrase;
Conference_Titel :
Future Information Technology and Management Engineering (FITME), 2010 International Conference on
Conference_Location :
Changzhou
Print_ISBN :
978-1-4244-9087-5
DOI :
10.1109/FITME.2010.5655611