DocumentCode :
3400420
Title :
Synthesis of monodispersed ORMOSIL nanoparticles and conjugation with DNA for gene therapy
Author :
Colaco, Russell ; Goncalves, M.C. ; Goncalves, L.M.D. ; Almeida, A.J. ; Martins, M. Barbara
Author_Institution :
Dept. de Eng. Quim. e Biologica, Tech. Univ. Lisbon, Lisbon, Portugal
fYear :
2011
fDate :
1-4 March 2011
Firstpage :
1
Lastpage :
4
Abstract :
Nanomedicine is an emerging new field combining nanotechnology and medicine. Silica nanoparticles are chemical and biologically inert, optically transparent and can be doped with imaging agents and/or functionalized to promote its conjugation with different therapeutic molecules. Silica nanoparticles can be engineered to improve diagnosis, treatment and follow-up of diseases. A combination of diagnosis devices and therapeutics (theranostics) would be beneficial for patients. In this work, ORMOSIL nanoparticles as non-viral vectors for gene delivery were prepared via a modified Stober sol-gel process directly with 3-aminopropyltriethoxysilane and tetraethylorthosilicate as precursors. Dynamic light scattering, transmission electron microscopy and Fourier transformed infrared spectroscopy were used to characterizing the hybrid nanospheres. Synthesis has been optimized and monodisperse spherical nanoparticles with desired size have been obtained. Nanoparticle-DNA complexes were successfully obtained at different ratios (nanoparticle/pDNA) and confirmed by agarose gel electrophoresis and ethidium bromide exclusion test.
Keywords :
DNA; Fourier transform spectra; biological techniques; biomedical materials; electrophoresis; gene therapy; infrared spectra; light scattering; nanomedicine; nanoparticles; organic compounds; silicon compounds; sol-gel processing; transmission electron microscopy; 3-aminopropyltriethoxysilane; DNA; Fourier transformed infrared spectroscopy; SiO2; agarose gel electrophoresis; dynamic light scattering; ethidium bromide exclusion test; gene delivery; gene therapy; hybrid nanospheres; modified Stober sol-gel process; monodisperse spherical nanoparticles; monodispersed ormosil nanoparticles; nanomedicine; nanoparticle-DNA complexes; non-viral vectors; tetraethylorthosilicate; transmission electron microscopy; DNA; Fluorescence; Gene therapy; Nanoparticles; Organic inorganic hybrid materials; Silicon compounds; DNA; ORMOSIL; bioconjugation; gene delivery; nanoparticles;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioengineering (ENBENG), 2011. ENBENG 2011. 1st Portuguese Meeting in
Conference_Location :
Lisbon
Print_ISBN :
978-1-4577-0522-9
Electronic_ISBN :
978-1-4577-0521-2
Type :
conf
DOI :
10.1109/ENBENG.2011.6026053
Filename :
6026053
Link To Document :
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