Title :
Mechanical Scan-Free Confocal Microscope by a Broadband Source and Two Balanced Wavelength-to-Space Transformations
Author :
Hsien-Yi Wang ; Bor-Shyh Lin ; Chien-Ya Lou ; Ming-Che Chan
Author_Institution :
Dept. of Nephrology, Chi-Mei Med. Center, Tainan, Taiwan
Abstract :
A new approach for performing confocal microscopy without no mechanical scanners was reported by a broadband light source, two similar 2-D wavelength-dispersive optical elements, and an uncooled charge-coupled device (CCD). In the new approach, 2-D sample structures were simultaneously and instantly mapped into the CCD by performing two balanced wavelength-to-space transformations and one space-to-wavelength transformation. Compared with a traditional mechanical-scanner-based confocal microscope, no mechanical scanners or additional post data-processing were required in this new approach. The sectioned images are directly shown in the CCD. The demonstrated balanced wavelength-encoded confocal microscope has a simple configuration and shows great potential for high imaging speed. It has the advantage of miniaturized packaging and other applications outside of the laboratory as well.
Keywords :
CCD image sensors; optical elements; optical microscopes; optical microscopy; 2D wavelength-dispersive optical elements; CCD; balanced wavelength-encoded confocal microscope; balanced wavelength-to-space transformations; broadband light source; charge-coupled device; confocal microscopy; high imaging speed; mechanical scan-free confocal microscope; miniaturized packaging; Charge coupled devices; Gratings; Image resolution; Microscopy; Optical microscopy; Optical reflection; Broadband Source; Confocal Microscope; Confocal microscope; Diffractive Optics; Wavelength Encoded; broadband source; diffractive optics; wavelength encoded;
Journal_Title :
Photonics Journal, IEEE
DOI :
10.1109/JPHOT.2015.2390617