Title :
Artificial cells containing genetically engineered E. coli DH5 cells for urea and ammonia removal in kidney and liver failure
Author :
Prakash, S. ; Chang, T.M.S.
Author_Institution :
Dept. of Biomed. Eng., McGill Univ., Montreal, Que., Canada
Abstract :
Describes a novel method of urea and ammonia removal using microencapsulated genetically engineered E. coli DH5 cells. In vitro, 100 mg of bacterial cells encapsulated in alginate-polylysine-alginate (APA) membrane, in their log phase state of growth, can lower 87.89±2.25% of the plasma urea within 20 minutes and 99.99% in 30 minutes. The same amount of encapsulated bacteria is also capable of lowering ammonia from 975.14±70.15 μM/L to 81.151±7.37 μM/L in 30 minutes. This new approach is significantly more efficient than the presently available methods of urea and ammonia removal
Keywords :
ammonia; artificial organs; biotechnology; cellular biophysics; genetics; kidney; liver; organic compounds; 20 min; 30 min; alginate-polylysine-alginate membrane; ammonia removal; artificial cells; genetically engineered E. coli DH5 cells; kidney failure; liver failure; log phase growth state; medical therapeutic technique; microencapsulated cells; plasma urea; urea removal; Assembly; Biomedical engineering; Biomembranes; Chemicals; Genetic engineering; In vitro; Liver; Microorganisms; Needles; Plasma chemistry;
Conference_Titel :
Engineering in Medicine and Biology Society, 1995., IEEE 17th Annual Conference
Conference_Location :
Montreal, Que.
Print_ISBN :
0-7803-2475-7
DOI :
10.1109/IEMBS.1995.579913
