Title :
Further studies on selection for viable cells in culture using cell sorter technology
Author :
Tanz, Warren S. ; Tsongalis, Gregory J. ; Lamber, W.C.
Author_Institution :
Dept. of Pathol., Univ. of Med. & Dentistry of New Jersey, Newark, NJ, USA
Abstract :
The authors have developed a two-channel method, using forward and lateral light scatter, for selecting live and nonviable cultured lymphoblastoid cells without exposing the cells to any dye. These parameters are related to cell size and cell granularity, both of which are quickly altered in cells which become nonviable as determined by dye exclusion (0.1% trypan blue). Using the nontoxic vital dye, fluorescein diacetate, the authors have also developed a system which only requires a single channel to unequivocally separate live and nonviable lymphoblastoid cells in culture.<>
Keywords :
biological techniques and instruments; cellular biophysics; light scattering; cell culture; cell granularity; cell size; cell sorter technology; cultured lymphoblastoid cells; dye exclusion; fluorescein diacetate; light scatter; trypan blue;
Conference_Titel :
Engineering in Medicine and Biology Society, 1988. Proceedings of the Annual International Conference of the IEEE
Conference_Location :
New Orleans, LA, USA
Print_ISBN :
0-7803-0785-2
DOI :
10.1109/IEMBS.1988.94529
