DocumentCode :
3420858
Title :
Optical beam guidance in monolithic polymer chips for miniaturized colorimetric assays
Author :
Grumann, M. ; Moser, I. ; Steigert, J. ; Riegger, L. ; Geipel, A. ; Kohn, C. ; Urban, G. ; Zengerle, R. ; Ducree, J.
Author_Institution :
IMTEK, Freiburg Univ., Germany
fYear :
2005
fDate :
30 Jan.-3 Feb. 2005
Firstpage :
108
Lastpage :
111
Abstract :
For the first time, we present a simple and robust optical concept to enable precise and sensitive read-out of colorimetric assays in flat lab-on-a-chip devices. The optical guidance of the probe beam through an incorporated measurement chamber to the detector is based on the total internal reflection at V-grooves in the polymer chip. This way, the optical path length through the flat measurement chamber and thus the performance of the measurements are massively enhanced compared to direct (perpendicular) beam incidence. This is demonstrated by a chip-based, colorimetric glucose-assay on serum. Outstanding features are an excellent reproducibility (CV= 1.91 %), a competitive lower limit of detection (cmin = 124 μM), and a high degree of linearity (R2 = 0.998) within a working range extending over nearly three orders of magnitude.
Keywords :
colorimetry; integrated optics; light reflection; optical polymers; substrates; V-grooves; chip-based colorimetric glucose-assay; direct beam incidence; flat lab-on-a-chip devices; measurement chamber; miniaturized colorimetric assays; monolithic polymer chips; optical beam guidance; optical guidance; optical path length; polymer chip; probe beam; total internal reflection; Lab-on-a-chip; Length measurement; Optical beams; Optical devices; Optical polymers; Optical reflection; Optical sensors; Probes; Robustness; Semiconductor device measurement;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Micro Electro Mechanical Systems, 2005. MEMS 2005. 18th IEEE International Conference on
ISSN :
1084-6999
Print_ISBN :
0-7803-8732-5
Type :
conf
DOI :
10.1109/MEMSYS.2005.1453879
Filename :
1453879
Link To Document :
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