Title :
Integrated on-line microdevice for proteomics
Author :
Tabuchi, M. ; Baba, Y.
Author_Institution :
Dept. of Molecular & Pharm. Biotechnol., Tokushima Univ., Japan
fDate :
30 Jan.-3 Feb. 2005
Abstract :
This paper reports an integrated online microdevice for protein analysis we developed. Our developed microdevice includes all steps of cell culture, pretreatment procedures and electrophoresis separation. In conventional method, pipetting and centrifugation are indispensable for washing cells, changing media and extraction of proteins during protein assay. Our developed system requires no external machine, such as centrifuge or pump. Our system can be free from troublesome pipetting. Utilizing the integrated online microdevice, dramatically reduced total analytical time (1 min per 12 samples) was achieved.
Keywords :
biological specimen preparation; microfluidics; molecular biophysics; proteins; cell culture; cell washing; centrifugation; electrophoresis separation; online microdevice; pipetting; pretreatment procedures; protein analysis; protein assay; protein extraction; proteomics; pump; Biotechnology; Chemical analysis; Chemistry; Electrokinetics; High temperature superconductors; Immune system; Pharmaceuticals; Protein engineering; Proteomics; System-on-a-chip;
Conference_Titel :
Micro Electro Mechanical Systems, 2005. MEMS 2005. 18th IEEE International Conference on
Print_ISBN :
0-7803-8732-5
DOI :
10.1109/MEMSYS.2005.1454019
