A world-to-chip microfluidic interconnection technology with dual functions of sample injection and sealing for a multichamber micro PCR chip

This paper presents a practical world-to-chip microfluidic interconnection technology with dual functions of sample injection and sealing for a multichamber Micro PCR (Polymerase Chain Reaction) chip. After sample injection and sealing, leakage test is conducted by elevating the temperature up to 100 °C for 30 min. No leakage flows are found during the test for 10 cartridges. In conclusion, we have introduced a simple and cheap microfluidic interconnection technology for both sample injection and sealing, which provides a zero dead volume, a zero leakage flow, and biochemical compatibility. Also, this world-to-chip interconnection technology enables one or more operators to interface between the micro world and real world easily by using conventional pipettes.