Title :
Quantitative determination of total alkaloid in Corydalis Ochotensis Turcz by colorimetry
Author :
Mao Meina ; Liu Yongqiang ; Zhou Mingjuan ; Chen Hongyan ; Zhao Chunfang
Author_Institution :
Dept. of Pharmacy, Jilin Univ., Changchun, China
Abstract :
A quantitative determination of the total alkaloid in Corydalis Ochotensis Turcz was established by acid dye colorimetry. The effects of wavelength, pH of the phosphate buffer and the quantity of acid dye were studied. Two different dyes were compared according to the yield of berberine hydrochloride. The method with bromophenol blue dye was proved to be more accurate, more stable with good reproducibility in testing experiments. So, it was fit for quantitative determination of total alkaloid in Corydalis Ochotensis Turcz.
Keywords :
biochemistry; chemical analysis; colorimetry; dyes; pH; acid dye colorimetry; berberine hydrochloride; bromophenol blue dye; corydalis ochotensis turcz; pH; phosphate buffer; quantitative determination; total alkaloid; Correlation; Educational institutions; Equations; Ethanol; Materials; Presses; Stability analysis; Acid dye colorimetry; Bromophenol blue; Corydalis Ochotensis Turcz; Total alkaloids;
Conference_Titel :
Human Health and Biomedical Engineering (HHBE), 2011 International Conference on
Conference_Location :
Jilin
Print_ISBN :
978-1-61284-723-8
DOI :
10.1109/HHBE.2011.6027890
