Title :
Biomolecular linear motors confined to move upon micropatterns on glass
Author :
Yoshida, Yumi ; Yokokawa, Ryuji ; Suzuki, Hiroaki ; Atsuta, Kyoko ; Fujita, Hiroyuki ; Takeuchi, Shoii
Author_Institution :
Center for Int. Res. on MicroMechatronics, Tokyo Univ., Japan
fDate :
30 Jan.-3 Feb. 2005
Abstract :
Biomolecular linear motor proteins - kinesin and microtubules - are finely patterned on a conventional, flat glass substrate using parylene lift-off process; these patterns are useful for single molecule analysis and biohybrid transportation. The patterning was performed from 5 μm - 40 μm in width with minimal nonspecific binding of the proteins. A conventional gliding assay was realized using this glass substrate and the behaviors of the single proteins were analyzed.
Keywords :
biomolecular electronics; glass; linear motors; micromotors; nanotechnology; proteins; 5 to 40 micron; biohybrid transportation; biomolecular linear motor proteins; glass micropatterns; glass substrate; gliding assay; kinesin; microtubules; nonspecific protein binding; parylene lift-off process; patterning; single molecule analysis; Biological materials; Glass; Microfluidics; Micromotors; Microscopy; Pattern analysis; Polymers; Proteins; Surface treatment; Transportation;
Conference_Titel :
Micro Electro Mechanical Systems, 2005. MEMS 2005. 18th IEEE International Conference on
Print_ISBN :
0-7803-8732-5
DOI :
10.1109/MEMSYS.2005.1454038
