The anticoagulant activity of polysaccharide and sulfate from corn-cob

Extracted the polysaccharide and sulfate from corn-cob, and approached the anticoagulant activity of them in vivo and in vitro in this study. Methods Water-soluble crude polysaccharide has been extracted by acid from corn-cod. Sulfation of ACC with chlorosulfonic acid-pyridine, and detection of sulfate content by Ion Chromatography; Determination the bleeding time(BT) and clotting time(CT) of small white mice. Result the Corn-cob polysaccharide extraction was 12.5g; Sulfated polysa-ccharide Sulfate was 9.86%; the BT and CT were prolonged in vivo with statistic significant difference (p<;0.01) except low-dose and mid-dose group were show Little effect (p>;0.05) Conclusion The anticoagulation effect of high-dose group ACC, ACP and ACS were showed in vivo and dose-dependent relation.