An effiecient way to extract pilose antler polypeptide and evaluation its role in the immune response

Author

Pan, F.G. ; Zhao, Y.Y. ; Zhu, Shuyuan ; Li, H.S. ; Fang, Zhou ; Qin, Y.N. ; Wu, Huwei ; Xing, H.Q. ; Pang, Yanwei ; Zhang, M.D.

Author_Institution

Lab. of Nutrition & Functional Food, Jilin Univ., Changchun, China

fYear

2011

fDate

19-22 Aug. 2011

Firstpage

434

Lastpage

437

Abstract

The present study is to find an efficient method for extracting pilose antler polypeptide (PAP), and the immunological function of PAP was systematically evaluated. Sephadex G-50 Molecular exclusion chromatography and CM-Sepharose Fast Flow ion exchange chromatography were used to extract PAP which is more efficient than traditional method. Spleen lymphocyte proliferation assays and cytokine IL-12 secretion assays were used to study the immunological function of PAP. The different concentrations of PAP (4.5 ug/ml, 5 ug/ml, 5.5 ug/ml and 6 ug/ml) have significantly advanced effect on the lymphocyte cell proliferation. The result indicated that PAP may have an important immunological function to improve hosts´ immunity in vivo.

Keywords

biochemistry; blood; cellular biophysics; chromatography; ion exchange; polymers; CM-sepharose fast flow ion exchange chromatography; Sephadex G-50 molecular exclusion chromatography; cytokine IL-12 secretion assays; immunological function; lymphocyte cell proliferation; pilose antler polypeptide; spleen lymphocyte proliferation assays; traditional method; Heating; Immune system; Peptides; Proteins; Purification; Rats; extraction; function; pilose antler polypeptide; purification;

fLanguage

English

Publisher

ieee

Conference_Titel

Human Health and Biomedical Engineering (HHBE), 2011 International Conference on

Conference_Location

Jilin

Print_ISBN

978-1-61284-723-8

Type

conf

DOI

10.1109/HHBE.2011.6027992

Filename

6027992