Title :
An effiecient way to extract pilose antler polypeptide and evaluation its role in the immune response
Author :
Pan, F.G. ; Zhao, Y.Y. ; Zhu, Shuyuan ; Li, H.S. ; Fang, Zhou ; Qin, Y.N. ; Wu, Huwei ; Xing, H.Q. ; Pang, Yanwei ; Zhang, M.D.
Author_Institution :
Lab. of Nutrition & Functional Food, Jilin Univ., Changchun, China
Abstract :
The present study is to find an efficient method for extracting pilose antler polypeptide (PAP), and the immunological function of PAP was systematically evaluated. Sephadex G-50 Molecular exclusion chromatography and CM-Sepharose Fast Flow ion exchange chromatography were used to extract PAP which is more efficient than traditional method. Spleen lymphocyte proliferation assays and cytokine IL-12 secretion assays were used to study the immunological function of PAP. The different concentrations of PAP (4.5 ug/ml, 5 ug/ml, 5.5 ug/ml and 6 ug/ml) have significantly advanced effect on the lymphocyte cell proliferation. The result indicated that PAP may have an important immunological function to improve hosts´ immunity in vivo.
Keywords :
biochemistry; blood; cellular biophysics; chromatography; ion exchange; polymers; CM-sepharose fast flow ion exchange chromatography; Sephadex G-50 molecular exclusion chromatography; cytokine IL-12 secretion assays; immunological function; lymphocyte cell proliferation; pilose antler polypeptide; spleen lymphocyte proliferation assays; traditional method; Heating; Immune system; Peptides; Proteins; Purification; Rats; extraction; function; pilose antler polypeptide; purification;
Conference_Titel :
Human Health and Biomedical Engineering (HHBE), 2011 International Conference on
Conference_Location :
Jilin
Print_ISBN :
978-1-61284-723-8
DOI :
10.1109/HHBE.2011.6027992
