Title :
Construction of prokaryotic vector of Timp-3
Author :
Duan Lianxin ; Sun Xin ; Wang Yan ; Xu Dan ; Guan Xingang ; Zheng Di
Author_Institution :
Clinical Lab. Med. Coll., Beihua Univ., Jilin, China
Abstract :
Purposes: constructing prokaryotic vector of Timp3 (tissue inhibitor of metalloproteinase-3). Methords: Total RNA of human placenta tissues were prepared, and the cDNA encoding Timp3 was got via RT-PCR. The gene was constructed into pMD18-T vector using the restriction enzyme of BamHI and XbaI. Results: By agrose gel electrophoresis and DNA sequencing, Timp3 gene was introduced into pMD18-T successly.
Keywords :
DNA; biochemistry; biological tissues; cellular biophysics; electrophoresis; enzymes; gels; genetics; molecular biophysics; BamHI enzyme; DNA sequencing; RNA; Timp3 gene; Xbal enzyme; agrose gel electrophoresis; gene construction; human placenta tissues; pMD18-T vector; prokaryotic vector construction; Biochemistry; DNA; Humans; Inhibitors; Muscles; RNA; Tumors; RT-PCR; Timp3; pMD18-T;
Conference_Titel :
Human Health and Biomedical Engineering (HHBE), 2011 International Conference on
Conference_Location :
Jilin
Print_ISBN :
978-1-61284-723-8
DOI :
10.1109/HHBE.2011.6028374
