A microfluidic-based platform for in vitro studies of cell signaling in blood vessels

Author

Riahi, R. ; Yang, Yi ; Kim, Heonhwan ; Jiang, L. ; Wong, P.K. ; Zohar, Yitshak

Author_Institution

Univ. of Arizona, Tucson, AZ, USA

fYear

2013

fDate

16-20 June 2013

Firstpage

293

Lastpage

296

Abstract

A microfluidic-based platform is developed for in vitro investigation of signaling within the blood-stream leading to preferential homing of specific cells such as white blood cells (WBCs) selectively to injured host organs. A microchannel has been coated with a monolayer of endothelial cells to mimic the microenvironment in blood vessels, while stable chemokine gradients have been generated locally at selected locations along the microchannel sidewalls. We demonstrate that both the chemokine gradients and the endothelial cells are simultaneously maintained during steady flow of cells as in the blood stream.

Keywords

bioMEMS; biochemistry; biological organs; biological techniques; blood vessels; cellular biophysics; microfabrication; microfluidics; molecular biophysics; monolayers; proteins; WBC; biological organs; blood stream; blood vessels; blood-stream; cell signaling; chemokine gradients; coating; endothelial cells; in vitro investigation; microchannel; microchannel sidewalls; microenvironment; microfluidic-based platform; monolayer; steady cell flow; white blood cells; Adhesives; Biomedical imaging; Blood vessels; In vitro; Microchannel; Microfluidics; Tumors; Microfluidic system; cell signaling; chemokine gradients; endothelial cells;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS & EUROSENSORS XXVII), 2013 Transducers & Eurosensors XXVII: The 17th International Conference on

Conference_Location

Barcelona

Type

conf

DOI

10.1109/Transducers.2013.6626760

Filename

6626760